hiscript ii 1st strand cdna synthesis kit (Vazyme Biotech Co)
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hiscript ii 1st strand cdna synthesis kit
Hiscript Ii 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 2179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hiscript+ii+1st+strand+cdna+synthesis+kit/HiScript+III+All-in-one+RT+SuperMix+Perfect+for+qPCR/pmc13010958-103-14-22
Average 99 stars, based on 2179 article reviews
Hiscript Ii 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 2179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hiscript+ii+1st+strand+cdna+synthesis+kit/HiScript+III+All-in-one+RT+SuperMix+Perfect+for+qPCR/pmc13010958-103-14-22
Average 99 stars, based on 2179 article reviews
hiscript ii 1st strand cdna synthesis kit - by Bioz Stars,
2026-09
99/100 stars
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Reverse Transcription:Article Title: Paeonol alleviates granulosa cell senescence in laying chickens via the PI3K/Akt/mTOR signaling pathway Article Snippet: Stained sections were subsequently examined and imaged using an Eclipse 80i light microscope (Nikon, Tokyo, Japan). .. Total RNA was extracted using TRIzol reagent (TaKaRa, Dalian, China) and reverse-transcribed using the Article Title: Miniature and versatile genome regulation TnpB-ωRNA toolkits facilitate cancer immunotherapy. Article Snippet: Quantitative RT‒PCR Total RNA was isolated from cells or tumor tissues, the latter of which were snap-frozen in liquid nitrogen and mechanically homogenized via TRIzol reagent (TaKaRa, 9109). .. Up to 1 μg of RNA was reverse transcribed via a Article Title: Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens Article Snippet: The concentration and purity of the RNA samples were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Fisher, USA). .. Subsequently, the isolated total RNA was reverse transcribed into cDNA employing the cDNA Synthesis:Article Title: Paeonol alleviates granulosa cell senescence in laying chickens via the PI3K/Akt/mTOR signaling pathway Article Snippet: Stained sections were subsequently examined and imaged using an Eclipse 80i light microscope (Nikon, Tokyo, Japan). .. Total RNA was extracted using TRIzol reagent (TaKaRa, Dalian, China) and reverse-transcribed using the Article Title: Miniature and versatile genome regulation TnpB-ωRNA toolkits facilitate cancer immunotherapy. Article Snippet: Quantitative RT‒PCR Total RNA was isolated from cells or tumor tissues, the latter of which were snap-frozen in liquid nitrogen and mechanically homogenized via TRIzol reagent (TaKaRa, 9109). .. Up to 1 μg of RNA was reverse transcribed via a Article Title: Intratumoral microenvironment remodeling by lncRNA ROLLCSC enhances lung adenocarcinoma progression Article Snippet: First, total RNA was isolated via the RNA-Easy Isolation Reagent (Vazyme, R701-01). .. The isolated RNA was subsequently converted into cDNA via the Article Title: Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens Article Snippet: The concentration and purity of the RNA samples were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Fisher, USA). .. Subsequently, the isolated total RNA was reverse transcribed into cDNA employing the Article Title: GPAT3 facilitates AHPND-causing Vibrio parahaemolyticus pathogenesis by driving lipid droplets accumulation in shrimp. Article Snippet: Acute hepatopancreatic necrosis disease (AHPND), caused by a specific strain of Vibrio parahaemolyticus (VpAHPND), disrupts host immune function by promoting the accumulation of lipid droplets (LDs).. However, the underlying mechanisms and physiological consequences of LDs accumulation during VpAHPND infection in shrimp remain poorly characterized.. In this study, we investigate the role of Penaeus vannamei glycerol-3-phosphate acyltransferase 3 (PvGPAT3), a key enzyme in triglyceride and LDs biosynthesis, during VpAHPND infection. Article Title: Harnessing GhGRDP1 natural variation for enhanced cotton seed yield: a rhamnose-dependent strategy in breeding Article Snippet: Total RNA was purified and eluted using the VAHTS mRNA Capture Beads 2.0 (N403-01, Vazyme). .. Subsequently, the 5′ RNA adapter (sequence: 5′-GUUCAGAGUUCUACAGUCCGAC-3′) was ligated to the purified mRNA using T4 RNA ligase (M0204S, New England Biolabs). cDNA synthesis was performed using the Article Title: Insights into SARS-CoV-2 replication control via targeting the host hijacking function of NSP12 Article Snippet: Total RNA was extracted using the RNA-easy Isolation Reagent (Vazyme, Nanjing, China), and its concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. The cDNA was synthesized using the Article Title: Hijacking innate immunity to enhance mRNA therapeutics by blocking IFN-P-body-XRN1 axis-mediated degradation. Article Snippet: Cell Counting Kit8 (CCK-8) (CK04-500) was from Dojindo. .. FastPure cell/tissue total RNA isolation kit V2 (RC11201), Isolation:Article Title: Intratumoral microenvironment remodeling by lncRNA ROLLCSC enhances lung adenocarcinoma progression Article Snippet: First, total RNA was isolated via the RNA-Easy Isolation Reagent (Vazyme, R701-01). .. The isolated RNA was subsequently converted into cDNA via the Article Title: Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens Article Snippet: The concentration and purity of the RNA samples were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Fisher, USA). .. Subsequently, the isolated total RNA was reverse transcribed into cDNA employing the Article Title: Hijacking innate immunity to enhance mRNA therapeutics by blocking IFN-P-body-XRN1 axis-mediated degradation. Article Snippet: Cell Counting Kit8 (CCK-8) (CK04-500) was from Dojindo. .. FastPure cell/tissue total RNA isolation kit V2 (RC11201), Synthesized:Article Title: GPAT3 facilitates AHPND-causing Vibrio parahaemolyticus pathogenesis by driving lipid droplets accumulation in shrimp. Article Snippet: Acute hepatopancreatic necrosis disease (AHPND), caused by a specific strain of Vibrio parahaemolyticus (VpAHPND), disrupts host immune function by promoting the accumulation of lipid droplets (LDs).. However, the underlying mechanisms and physiological consequences of LDs accumulation during VpAHPND infection in shrimp remain poorly characterized.. In this study, we investigate the role of Penaeus vannamei glycerol-3-phosphate acyltransferase 3 (PvGPAT3), a key enzyme in triglyceride and LDs biosynthesis, during VpAHPND infection. Article Title: Insights into SARS-CoV-2 replication control via targeting the host hijacking function of NSP12 Article Snippet: Total RNA was extracted using the RNA-easy Isolation Reagent (Vazyme, Nanjing, China), and its concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. The cDNA was synthesized using the Sequencing:Article Title: Harnessing GhGRDP1 natural variation for enhanced cotton seed yield: a rhamnose-dependent strategy in breeding Article Snippet: Total RNA was purified and eluted using the VAHTS mRNA Capture Beads 2.0 (N403-01, Vazyme). .. Subsequently, the 5′ RNA adapter (sequence: 5′-GUUCAGAGUUCUACAGUCCGAC-3′) was ligated to the purified mRNA using T4 RNA ligase (M0204S, New England Biolabs). cDNA synthesis was performed using the Purification:Article Title: Harnessing GhGRDP1 natural variation for enhanced cotton seed yield: a rhamnose-dependent strategy in breeding Article Snippet: Total RNA was purified and eluted using the VAHTS mRNA Capture Beads 2.0 (N403-01, Vazyme). .. Subsequently, the 5′ RNA adapter (sequence: 5′-GUUCAGAGUUCUACAGUCCGAC-3′) was ligated to the purified mRNA using T4 RNA ligase (M0204S, New England Biolabs). cDNA synthesis was performed using the |